Plant Gender Detection Test
Test Summary: Real-time quantitative PCR (qPCR) assay using a multiplex system of primers to detect male associated DNA within the leaf sample.
Methodology: DNA was extracted from the germinated hemp cotyledons or mature leaf and then tested by a multiplexed qPCR for specific DNA sequence targeting a portion of the Y Chromosome associated with male plants. An internal control is used to verify DNA extraction process. Each run contains a non-template negative control as well as a positive control for male associated DNA.
Minimum Sample Size Requirements:
One intact leaf from as early as the second set of true leaves
Collection Container Requirements: Clean container such as sealable mylar bag or a screw top vial or test tube.
Turn Around Time: 2 to 5 business days from lab receipt of sample.