Test Summary: Real-time quantitative PCR (qPCR) assay using a multiplex system of primers to detect male associated DNA within the leaf sample.  

 

Methodology:  DNA was extracted from the germinated hemp cotyledons or mature leaf and then tested by a multiplexed qPCR for specific DNA sequence targeting a portion of the Y Chromosome associated with male plants.  An internal control is used to verify DNA extraction process. Each run contains a non-template negative control as well as a positive control for male associated DNA.

 

Minimum Sample Size Requirements: 

• One intact leaf from as early as the second set of true leaves 

 

Collection Container Requirements:  Clean container such as sealable mylar bag or a screw top vial or test tube.  

 

Turn Around Time:  2 to 5 business days from lab receipt of sample. 

 

Cost: $10.00